Toshihiro Shioda, MD, PhD

Massachusetts General Hospital

Academic Titles

Associate Professor, Medicine, Harvard Medical School

Research Program Affiliation

Member, Breast Cancer

Research Abstract

My laboratory has been working on the following areas of studies.

(i) Roles and mechanisms of actions of estrogens and antiestrogens in human breast cancer. [MCF-7 cell culture and its monoclonal sublines with different sensitivities to antiestrogens; The TRC shRNA lentivirus library; high-throughput RNAi knockdown screening; Affymetrix microarray]

(ii) Pharmacogenomics and Toxicogenomics of estrogens and xenobiotic environmental estrogenic substances. [MCF-7 cell culture; Affymetrix microarray, high-throughput RT-qPCR; computational biology]

(iii) Exposure of fetuses to the environmental substances and bioactive food compoents during their early stages of developement: Effects on epigenetic gene regulation and breast cancer risk. [rat fetuses, chicken embryos, zebrafish embryos, DNA methylation, bisulfite sequencing, ultradeep parallel sequencing, Affymetrix microarray, high-throughput RT-qPCR]

(iv) Toxicological servey on the environmental contamination by estrogenic xenoestrogens. [MCF-7 cell culture, RT-qPCR]